TY - JOUR AU - Carmen Ruiz, Doris Esenarro, Raul Mendez, Elizabeth Luz Segovia Aranibar, Samuel Reyna Mandujano, PY - 2020/11/02 Y2 - 2024/03/29 TI - Isolation, identification, cloning and phylogenetic analysis of the micro algae Prorocentrum minimum (Pavillard) J. Schiller 1933 Isolated from the Bay of Callao - Peru JF - PalArch's Journal of Archaeology of Egypt / Egyptology JA - J Arch.Egyptol VL - 17 IS - 9 SE - DO - UR - https://www.archives.palarch.nl/index.php/jae/article/view/4013 SP - 1836 - 1849 AB - <p>Harmful Algal Blooms (HAB) or Red Tides are water discolorations visible to the naked<br>eye due to the proliferation of one or more planktonic microorganisms such as microalgae. These<br>can reach high levels and produce adverse effects on human health, as well as cause damage to<br>other marine organisms near the coast. For this reason, there was interest in isolating, identifying<br>and making a phylogenetic study of the species Prorocentrum minimum found in the Bay of<br>Callao, Peru. Several samples of seawater were taken for their subsequent morphological<br>identification, and the technique of purification of the microalgae was carried out. At the same<br>time, a standard of Prorocentrum minimum was acquired from the Provasoli- Guillard National<br>Center for Culture of Marine Phytoplankton (CCMP), USA. It was carried out the growth curve<br>for the concentration of microalgal density, later it was carried out the DNA extraction and the<br>molecular phylogeny from the sequences of the ribosome LSU rRNA subunits of the species<br>Prorocentrum minimum. P. minimum and P gracile were identified, which were close epibenthic<br>species because they were more recent evolutionarily. Likewise, to confirm the presence of some<br>marine biotoxin absence in Prorocentrum minimum culture, it was established a quantitative<br>analysis of the animal's dose response in the mouse bioassay. It was possible to obtain the<br>purification of Prorocentrum minimum culture and to standardize the work protocol; it was<br>confirmed the phylogeny of microalgae species. It was not possible to obtain DSP toxin from<br>microalgae culture.</p> ER -